[Optimization of a HPLC determination method for Evodia rutaecarpa].

Area of Science:

• Pharmacognosy
• Analytical Chemistry
• Phytochemistry

Background:

• Evodia rutaecarpa is a traditional medicinal herb.
• Key bioactive compounds include limonin, evodiamine, and rutaecarpine.
• Accurate quantification of these compounds is crucial for quality control.

Purpose of the Study:

• To optimize a High-Performance Liquid Chromatography (HPLC) method for determining limonin, evodiamine, and rutaecarpine in Evodia rutaecarpa.
• To establish a reliable analytical method for quality assessment of the herb.

Main Methods:

• Optimization of HPLC parameters including mobile phase composition (acetonitrile-tetrahydrofuran-0.02% H3 PO4), detection wavelength (220 nm), and flow rate (1.0 mL/min).
• Validation of the method for linearity, recovery, and reproducibility (RSD).

Main Results:

• The optimized HPLC method achieved excellent separation of limonin, evodiamine, and rutaecarpine.
• Linear ranges were established as 0.1968-3.936 µg for limonin, 0.1536-3.072 µg for evodiamine, and 0.0974-1.948 µg for rutaecarpine.
• Average recoveries were high (97.8%, 100.7%, 98.4%) with low relative standard deviations (RSD: 1.7%, 1.3%, 1.1%).

Conclusions:

• The developed HPLC method is accurate, reproducible, and efficient.
• This method provides a robust tool for enhancing the quality control of Evodia rutaecarpa.
• Reliable quantification of key compounds ensures the consistency and efficacy of herbal products.