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Physical mapping and molecular cloning of mung bean yellow mosaic virus DNA
T Morinaga1, M Ikegami, K Miura
1Biotechnology Research Laboratory, Teijin Tokyo Research Center, Japan.
Intervirology
|January 1, 1990
Abstract:
Viral single-stranded DNA of mung bean yellow mosaic virus (MYMV) was converted to the double-stranded state in vitro, and physical mapping was carried out. The genome of MYMV was found to consist of two major components (designated as DNA 1 and DNA 2). In addition, some minor components were detected. Molecular cloning of the major components was carried out, using in vitro double-stranded DNA and replicative intermediate DNAs. DNA 1 is about 2.72 and DNA 2 about 2.67 kilobase pairs. No similarities were observed when the two restriction maps of DNA 1 and 2 were compared.