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Updated: Jun 1, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

Crystallizing membrane proteins for structure-function studies using lipidic mesophases.

Martin Caffrey1

  • 1Membrane Structural and Functional Biology Group, School of Biochemistry and Immunology, and School of Medicine, Trinity College Dublin, Ireland. martin.caffrey@tcd.ie

Biochemical Society Transactions
|May 24, 2011
PubMed
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The lipidic cubic phase method simplifies membrane protein crystallization, overcoming handling challenges with new tools and screens. This technique is increasingly vital for structural biology, particularly for G-protein-coupled receptors.

Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • The lipidic cubic phase method is a powerful technique for membrane protein crystallization.
  • Recent successes, especially with G-protein-coupled receptors, highlight its potential.
  • Adoption is hindered by perceived difficulties in handling the mesophase and crystals.

Purpose of the Study:

  • To address the challenges associated with the lipidic cubic phase method.
  • To present advancements making the method more user-friendly and accessible.
  • To showcase the method's applicability to diverse membrane proteins.

Main Methods:

  • Development of tools for pico- to nano-litre volume mesophase handling for screening.
  • Introduction of specialized glass crystallization plates for enhanced optical quality.

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Crystallization of Membrane Proteins in Lipidic Mesophases
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Crystallization of Membrane Proteins in Lipidic Mesophases

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Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
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Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

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Last Updated: Jun 1, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
18:45

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Published on: September 2, 2012

  • Implementation of lipid and precipitant screens for rational crystallogenesis.

    • Main Results:

    • The lipidic cubic phase method has yielded six new G-protein-coupled receptor structures recently.
    • Advancements have made the method applicable to a wider range of membrane protein types and sizes.
    • The developed tools and screens facilitate efficient crystallization screening and data collection.

    Conclusions:

    • The lipidic cubic phase method is becoming an accepted and generalizable technique in membrane structural biology.
    • Despite challenges, ongoing developments are making the method more user-friendly.
    • Further improvements are needed to fully realize the method's potential.