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Recombination between viral and cellular sequences generates transforming sarcoma virus

L H Wang, C C Halpern, M Nadel

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1978
    PubMed

    Insights

    Recovered avian sarcoma viruses (rASVs) reacquire sarcoma-specific RNA sequences, including novel ones, within the src region. These sequences are crucial for the transforming function of these viruses.

    Area of Science:

    • Molecular Biology
    • Virology
    • Oncology

    Background:

    • Rous sarcoma virus (RSV) causes tumors and has transformation-defective (td) mutants.
    • Recovered avian sarcoma viruses (rASVs) arise from td mutants and regain transforming ability.

    Purpose of the Study:

    • To compare the RNA sequences of rASVs, td mutants, and SR-A RSV.
    • To map the acquired RNA sequences responsible for transforming function.

    Main Methods:

    • Oligonucleotide fingerprinting of viral RNA.
    • Comparison of RNA sequences between different virus strains.

    Main Results:

    • SR-A RNA contains six sarcoma-specific oligonucleotides; td mutants lack three to six of these.
    • All six rASV isolates regained these six oligonucleotides.
    • Most rASVs possess three new oligonucleotides not found in SR-A or td mutants, located within the src region.

    Conclusions:

    • Acquired RNA sequences in rASVs are mapped to the src region and segregate with transforming function.
    • These sequences include both novel and SR-A identical sequences, indicating cellular acquisition and recombination.

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