Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Resolution of cytochrome oxidase into two component complexes.

M Fry, H Vande Zande, D E Green

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1978
    PubMed
    Summary

    Cytochrome c oxidase, a key enzyme, is composed of two distinct complexes. This discovery reveals separate functions for electron transfer and ion transfer, impacting our understanding of energy coupling mechanisms.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A unified call to action from Australian nursing and midwifery leaders: ensuring that Black lives matter.

    Contemporary nurse·2020
    Same author

    Ambiguity hindering self-management and prevention of osteoporosis in post-menopausal women.

    Archives of osteoporosis·2020
    Same author

    Components of Resistance to Rhizoctonia solani Associated with Two Tall Fescue Cultivars.

    Plant disease·2019
    Same author

    The Structure Of The Eysenck Personality Inventory: A Comparison Between Simple And More Complex Analyses Of A Multiple Scale Questionnaire.

    Multivariate behavioral research·2016
    Same author

    Marrow adipogenesis and bone loss that parallels estrogen deficiency is slowed by low-intensity mechanical signals.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2015
    Same author

    Effect of online diabetes training for hospitalists on inpatient glycaemia.

    Diabetic medicine : a journal of the British Diabetic Association·2013

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Enzyme kinetics

    Background:

    • Cytochrome c oxidase (EC 1.9.3.1) is crucial for cellular respiration.
    • Previous studies suggested a single complex for its functions.

    Purpose of the Study:

    • To investigate the structural and functional components of cytochrome c oxidase.
    • To elucidate the role of different protein complexes within cytochrome c oxidase.

    Main Methods:

    • Enzyme complex resolution techniques.
    • Spectroscopic analysis (fluorescence induction).
    • Protein characterization.

    Main Results:

    • Cytochrome c oxidase resolved into two complexes of unequal molecular weight.

    Related Experiment Videos

  • The larger complex facilitates electron transfer.
  • The smaller complex mediates cation-dependent fluorescence induction, indicating ion transfer capabilities.

    • Conclusions:

    • Cytochrome c oxidase possesses a duplex nature with distinct functional roles.
    • This structural duality has significant implications for understanding energy coupling mechanisms in biological systems.