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Magnetic separation of cells from developing embryoid bodies using magnetite cationic liposomes
Masanobu Horie1, Akira Ito, Takeshi Maki
1Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Journal of Bioscience and Bioengineering
|May 24, 2011
Abstract:
Embryoid bodies resemble post-implantation egg-cylinder stage embryos and are used to differentiate embryonic stem cells in vitro. In this study, we enriched mouse vasa homolog-positive germ cells from embryoid bodies after 8d of differentiation using a magnetic separation method with magnetite cationic liposomes.
