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Updated: Jun 1, 2026

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
Published on: February 3, 2022
DNA polymerases and repair synthesis in NER in human cells
1Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, UK. a.r.lehmann@sussex.ac.uk
Abstract:
The late steps of nucleotide excision repair, following incisions to remove the damaged section of DNA, comprise repair synthesis and ligation. In vitro and in vivo studies have shown the size of the repaired patch to be about 30 nucleotides. In vitro studies implicated the replicative polymerases in repair synthesis, but recent in vivo data have shown that several DNA polymerases and ligases are involved in these steps in human cells.
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