Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generating exosome subtypes: diverse membrane origins and mergers.

Trends in cell biology·2026
Same author

SET1B Drives Sustained HIF activity and Disease Progression in Clear Cell Renal Cell Carcinoma.

Cancer research·2026
Same author

Editorial Expression of Concern: Direct targeting of Sec23a by miR-200s influences cancer cell secretome and promotes metastatic colonization.

Nature medicine·2026
Same author

A systems-based approach to uterine fibroids identifies differential splicing associated with abnormal uterine bleeding.

Communications medicine·2025
Same author

Tumor-specific CD8 T cell characterization in HR<sup>+</sup> breast cancer reveals an impaired antitumoral response in patients with lymph node metastasis.

Cell reports. Medicine·2025
Same author

Amyloid-β disrupts APP-regulated protein aggregation and dissociation from recycling endosomal membranes.

The EMBO journal·2025

Related Experiment Video

Updated: Jun 1, 2026

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
09:19

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis.

Rebecca A Dragovic1, Christopher Gardiner, Alexandra S Brooks

  • 1Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.

Nanomedicine : Nanotechnology, Biology, and Medicine
|May 24, 2011
PubMed
Summary

This study introduces fluorescence nanoparticle tracking analysis (NTA) for sizing and phenotyping cellular vesicles. NTA offers higher sensitivity than flow cytometry for detecting these important disease biomarkers.

More Related Videos

Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis
08:52

Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis

Published on: November 17, 2021

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing
12:01

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Published on: October 19, 2014

Related Experiment Videos

Last Updated: Jun 1, 2026

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
09:19

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis
08:52

Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis

Published on: November 17, 2021

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing
12:01

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Published on: October 19, 2014

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Cell Biology

Background:

  • Cellular microvesicles and nanovesicles (exosomes) play roles in disease and hold potential as biomarkers.
  • Current technological limitations hinder the development of vesicle-based diagnostics.
  • Accurate measurement of vesicle size and phenotype is crucial for biomarker discovery.

Purpose of the Study:

  • To evaluate fluorescence nanoparticle tracking analysis (NTA) for rapid sizing and phenotyping of cellular vesicles.
  • To compare the sensitivity of NTA with conventional flow cytometry for vesicle measurement.
  • To demonstrate the capability of NTA for specific vesicle phenotyping.

Main Methods:

  • Utilized fluorescence nanoparticle tracking analysis (NTA) to visualize and track individual vesicles via light scattering.
  • Employed Brownian motion analysis within NTA software to determine vesicle size and concentration.
  • Combined NTA with fluorescence measurement using antibody-conjugated quantum dots for vesicle labeling.

Main Results:

  • NTA successfully measured cellular vesicles as small as approximately 50 nm.
  • NTA demonstrated significantly higher sensitivity compared to conventional flow cytometry (lower limit ~300 nm).
  • Fluorescence-based NTA enabled specific phenotyping of labeled vesicles.

Conclusions:

  • Fluorescence NTA is a sensitive and rapid technology for sizing and phenotyping cellular vesicles.
  • NTA surpasses conventional flow cytometry in detecting smaller vesicles, expanding biomarker potential.
  • This method facilitates the characterization of vesicles for diagnostic and research applications.