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11:22
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
Published on: December 1, 2017
Genetic assembly tools for synthetic biology
Billyana Tsvetanova1, Lansha Peng, Xiquan Liang
1Life Technologies Corporation, Carlsbad, California, USA.
Methods in Enzymology
|May 24, 2011
Summary
Researchers developed new protocols for seamless DNA assembly, enabling flexible genetic engineering across a wide size range. This advancement supports complex biological system redesign and gene expression fine-tuning.
Area of Science:
- Synthetic Biology
- Molecular Biology
- Genetic Engineering
Background:
- Genome sequencing projects have expanded genetic bioengineering applications.
- Current DNA assembly methods lack robustness for diverse fragment sizes and flexibility for modifications.
Purpose of the Study:
- To present novel protocols for seamless, simultaneous, and flexible DNA assembly.
- To enable efficient assembly of genetic material across a wide size dynamic range (10s to 100,000s base pairs).
Main Methods:
- Developed protocols for in vitro and in vivo DNA assembly.
- Included methods for DNA fragments with or without homology at their ends.
- Introduced a novel site-directed mutagenesis approach enhanced by in vitro recombineering.
Main Results:
- Achieved seamless, simultaneous, flexible, and highly efficient assembly of genetic material.
- Demonstrated assembly across a wide size dynamic range.
- Enabled modifications without complicated cloning strategies.
Conclusions:
- The presented protocols offer a robust and flexible solution for genetic engineering.
- These advancements facilitate the integrated analysis of complex pathways and redesign of biological systems.
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03:36Genome Annotation and Assembly
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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