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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 1, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Magnetic microparticle-based multiplexed DNA detection with biobarcoded quantum dot probes.

Dong-shan Xiang1, Guo-ping Zeng, Zhi-ke He

  • 1Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

Biosensors & Bioelectronics
|May 24, 2011
PubMed
Summary

We developed a novel biobarcode assay using quantum dots (QDs) and magnetic microparticles (MMPs) for simultaneous DNA detection. This method efficiently detects human papillomavirus (HPV) DNA with high sensitivity and speed.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Diagnostics

Background:

  • Multiplex DNA detection is crucial for diagnosing infectious diseases and genetic disorders.
  • Existing biobarcode assays can be time-consuming and complex to prepare.

Purpose of the Study:

  • To develop a novel, rapid, and sensitive multiplex DNA detection method.
  • To utilize biobarcoded CdSe/ZnS quantum dots (QDs) and magnetic microparticles (MMPs) for simultaneous detection of human papillomavirus (HPV) DNA.

Main Methods:

  • Functionalized MMPs with capture DNA for HPV-16 and HPV-18.
  • Conjugated QDs with probe DNA and fluorescent labels (FAM/Rox).
  • Performed one-step hybridization, magnetic separation, and fluorescence detection.

Main Results:

  • Achieved linear fluorescence dependence on target DNA concentration (8 × 10⁻¹¹ to 8 × 10⁻⁹ M).
  • Reached detection limits of 7 × 10⁻¹¹ M for HPV-16 and 6 × 10⁻¹¹ M for HPV-18.
  • Demonstrated advantages over other biobarcode assays, including shorter preparation and analytical times.

Conclusions:

  • The QD-MMP based biobarcode assay is a simple, accurate, and efficient method for multiplex DNA detection.
  • This approach offers faster binding kinetics and reduced analytical time compared to existing methods.
  • The developed assay shows significant potential for clinical diagnostics and molecular biology research.