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Single unit recording from olfactory cilia.

S Frings1, B Lindemann

  • 1Department of Physiology, Universität des Saarlandes, Homburg/Saar, Federal Republic of Germany.

Biophysical Journal
|May 1, 1990
PubMed
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This study introduces a simple method to record olfactory neuron signals using a patch pipette on sensory cilia. This technique achieves picomolar odorant detection with minimal disturbance to the neuron.

Area of Science:

  • Neuroscience
  • Olfactory Receptor Cell Physiology

Background:

  • Olfactory receptor neurons possess sensory cilia crucial for odor detection.
  • Current methods for recording olfactory neuron activity include in-situ electrodes and patch-clamp on isolated cells, each with limitations.

Purpose of the Study:

  • To develop a technically simple method for recording olfactory neuron action potentials.
  • To assess the efficacy of a novel patch-pipette technique for olfactory cilia in detecting odorant thresholds.

Main Methods:

  • A patch pipette was used to gently pull sensory cilia from olfactory receptor cells into the pipette, positioned above the olfactory mucosa.
  • Electrical recordings were made without a tight seal, capturing current transients from action potentials.
  • The method was compared to traditional single-unit recordings and patch-clamp on isolated cells.

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Main Results:

  • The described method allows for the recording of current transients driven by action potentials from olfactory neurons.
  • The pipette covers less than 5% of the neuron's apical surface, ensuring minimal disturbance.
  • The neuron remains in situ with cilia partially covered by mucus, enabling odorant detection thresholds in the picomolar range.

Conclusions:

  • This patch-pipette technique offers a simple and minimally invasive approach to study olfactory neuron activity.
  • The method provides high sensitivity, achieving picomolar odorant detection thresholds.
  • It serves as a viable alternative to existing electrophysiological recording techniques for olfactory research.