Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals01:17

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging cell phone radiation in tissue mimics with hyperpolarized low-field MRI.

Science advances·2026
Same author

What Is the Information Content of EPR Oximetry?

Advances in experimental medicine and biology·2026
Same author

Electron paramagnetic resonance detection of superoxide in a murine model of acute lung injury.

Discover imaging·2025
Same author

Have We Been Teaching Continuous Wave EPR Correctly?

Journal of chemical education·2025
Same author

Compact 1 GHz electron paramagnetic resonance spectrometer and imager.

The Review of scientific instruments·2025
Same author

Comparison of 10 mm and 30 mm Diameter Surface Coil Resonators for 1 GHz EPR.

Applied magnetic resonance·2025

Related Experiment Video

Updated: Jun 1, 2026

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

A Wire Crossed-Loop-Resonator for Rapid Scan EPR.

George A Rinard1, Richard W Quine, Joshua R Biller

  • 1Department of Electrical Engineering, University of Denver.

Concepts in Magnetic Resonance. Part B, Magnetic Resonance Engineering
|May 24, 2011
PubMed
Summary
This summary is machine-generated.

A novel crossed-loop resonator (CLR) was developed for rapid scan in vivo electron paramagnetic resonance (EPR) imaging. This design effectively isolates signals from radiofrequency source noise, improving image quality.

More Related Videos

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

Related Experiment Videos

Last Updated: Jun 1, 2026

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

Area of Science:

  • Biophysics
  • Magnetic Resonance Imaging
  • Spectroscopy

Background:

  • Rapid scan electron paramagnetic resonance (EPR) imaging requires specialized resonators for in vivo applications.
  • Existing resonator designs face challenges with radiofrequency (RF)/microwave source noise and eddy currents, impacting image quality.
  • Open resonator designs are necessary for physiological support during animal imaging.

Purpose of the Study:

  • To design and construct a crossed-loop resonator (CLR) for VHF frequencies (near 250 MHz) suitable for rapid scan in vivo EPR imaging.
  • To minimize RF/microwave source noise and eddy current interference in rapid scan EPR.
  • To achieve a resonator design with sufficient bandwidth for rapidly changing signals.

Main Methods:

  • Construction of a crossed-loop resonator (CLR) using fine AWG 38 wire to minimize eddy currents.
  • Implementation of an open resonator design for improved animal accessibility.
  • Testing and characterization of resonator performance, including isolation from RF source noise and bandwidth.

Main Results:

  • Achieved significant isolation from RF source noise: 44 dB and 47 dB in two different resonator designs.
  • Minimized eddy currents by using fine wire, which also lowered resonator Q, increasing bandwidth.
  • The CLR design addressed key challenges in rapid scan EPR, including source noise and eddy currents.

Conclusions:

  • The developed crossed-loop resonator (CLR) is effective for rapid scan in vivo EPR imaging at VHF frequencies.
  • The CLR design successfully mitigates RF source noise and eddy current artifacts, enhancing image quality.
  • This resonator technology advances the capabilities of in vivo EPR imaging for biological research.