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Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes.
Babu Joseph1, Supriya Upadhyaya, Pramod Ramteke
1Department of Microbiology and Microbial Technology, College of Biotechnology and Allied Sciences, Allahabad Agricultural Institute-Deemed University, Uttar Pradesh, Allahabad-211007, India.
Enzyme Research
|May 24, 2011
Summary
Cold active lipase production was optimized using agroindustrial residues like groundnut oil cake via semisolid state fermentation. This cost-effective method using Micrococcus roseus yields enzymes active at low temperatures.
Area of Science:
- Biotechnology
- Enzyme Technology
- Microbial Fermentation
Background:
- Cold active enzymes are crucial for low-temperature industrial processes.
- Agroindustrial residues offer sustainable substrates for microbial fermentation.
- Semisolid state fermentation (SSF) is an efficient method for enzyme production.
Purpose of the Study:
- To optimize the production of cold active lipase using agroindustrial residues via SSF.
- To characterize the activity and stability of the produced cold active lipase.
- To explore the potential for cost-effective, large-scale lipase production.
Main Methods:
- Isolation of Micrococcus roseus from Gangotri glacier soil samples.
- Optimization of SSF using various agroindustrial substrates, focusing on groundnut oil cake (GOC).
- Supplementation studies with carbon (glucose) and nitrogen (ammonium nitrate) sources.
- Investigating the effect of triglycerides on lipase production.
- Characterization of enzyme activity and stability at different temperatures and pH levels.
Main Results:
- Groundnut oil cake (GOC) as a substrate yielded maximal lipase production at 15°C within 48 hours.
- Supplementation with 1% glucose and 2% ammonium nitrate significantly enhanced lipase yield.
- Triglyceride addition (0.5%) repressed lipase production.
- A 1:1 mixture of GOC and mustard oil cake (MOC) further improved production.
- The cold active lipase showed optimal activity at 10-15°C, stability below 30°C, optimum activity at pH 8, and >60% stability at pH 9.
Conclusions:
- Agroindustrial wastes, particularly GOC and MOC mixtures, are effective substrates for cold active lipase production via SSF.
- Optimization of nutrient supplementation enhances enzyme yield.
- The characterized cold active lipase is suitable for low-temperature applications.
- SSF utilizing agroindustrial waste presents a viable strategy for cost-effective, large-scale lipase production.
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