Related Experiment Video
Updated: Jun 1, 2026

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
Published on: March 29, 2018
Confocal microscopy for modeling electron microbeam irradiation of skin
John H Miller1, William B Chrisler, Xihai Wang
1School of Electrical Engineering and Computer Science, Washington State University Tri-Cities, 2710 University Drive, Richland, WA 99354, USA. jhmiller@tricity.wsu.edu
Abstract:
For radiation exposures employing targeted sources such as particle microbeams, the deposition of energy and dose will depend on the spatial heterogeneity of the sample. Although cell structural variations are relatively minor for two-dimensional cell cultures, they can vary significantly for fully differentiated tissues. Employing high-resolution confocal microscopy, we have determined the spatial distribution, size, and shape of epidermal keratinocyte nuclei for the full-thickness EpiDerm™ skin model (MatTek, Ashland, VA). Application of these data to calculate the microdosimetry and microdistribution of energy deposition by an electron microbeam is discussed.
More Related Videos
08:29Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells
Published on: October 17, 2025
08:18Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
Published on: September 5, 2017
Related Concept Videos
Confocal Fluorescence Microscopy
Overview of Electron Microscopy