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Updated: Jun 1, 2026
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Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
Published on: April 7, 2023
[Morphological characteristics of human adipose-derived stem cells]
Xu He1, Xufang Yang, Lihong Zhang
1Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China.
Summary
Researchers isolated and cultivated human adipose-derived stem cells (hADSCs), confirming their unique immunophenotypes and ultrastructure. These findings provide a morphological basis for hADSC applications in regenerative medicine.
Area of Science:
- Stem Cell Biology
- Tissue Engineering
- Regenerative Medicine
Context:
- Human adipose-derived stem cells (hADSCs) are multipotent mesenchymal stem cells with significant therapeutic potential.
- Characterizing hADSCs is crucial for their effective application in clinical settings.
- Standardized isolation and cultivation protocols are essential for obtaining pure and viable hADSC populations.
Purpose:
- To isolate and culture hADSCs from adipose tissue.
- To characterize the morphological, immunophenotypic, and ultrastructural properties of hADSCs.
- To establish a foundation for the clinical application of hADSCs.
Summary:
- hADSCs were isolated using collagenase digestion and cultured using adherence and monoclonal methods.
- Immunophenotyping via flow cytometry revealed hADSCs are positive for CD29, CD44, CD90, CD105, CD166 and negative for CD31, CD45, HLA-DR.
- Cytochemical and ultrastructural analyses confirmed distinct hADSC morphology, including abundant organelles and microvilli.
Impact:
- This study provides a comprehensive morphological and immunophenotypic characterization of hADSCs.
- The findings validate hADSCs as a promising cell source for regenerative medicine.
- Establishing clear morphological criteria aids in quality control and therapeutic development using hADSCs.
