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Cytoplasmic acidification and activation of Na+/H+ exchange during regulatory volume decrease in Ehrlich ascites

A Livne1, E K Hoffmann

  • 1Institute of Biological Chemistry, University of Copenhagen, Denmark.

The Journal of Membrane Biology
|March 1, 1990
PubMed
Summary
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Cytoplasmic acidification activates the Na+/H+ exchanger during regulatory volume decrease (RVD) in tumor cells. This activation limits RVD, but inhibiting the exchanger enhances the volume regulation response.

Area of Science:

  • Cellular physiology
  • Biochemistry
  • Oncology

Background:

  • Regulatory volume decrease (RVD) is a crucial cellular process for maintaining cell volume homeostasis.
  • Ehrlich ascites tumor cells are a model system for studying cell volume regulation.

Purpose of the Study:

  • To investigate the role of intracellular pH changes and the Na+/H+ exchanger in RVD of Ehrlich ascites tumor cells.
  • To determine the impact of Na+/H+ exchanger activity on the extent of RVD.

Main Methods:

  • Measurement of intracellular pH using a fluorescent pH indicator.
  • Assessment of regulatory volume decrease (RVD) in Ehrlich ascites tumor cells.
  • Manipulation of Na+/H+ exchanger activity using amiloride and Na+-free medium.

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Main Results:

  • Ehrlich ascites tumor cells showed cytoplasmic acidification during RVD.
  • This acidification activated the Na+/H+ exchanger with a set point around pH 7.0.
  • Inhibition of the Na+/H+ exchanger enhanced the RVD response, indicating incomplete volume regulation when the exchanger is active.

Conclusions:

  • Intracellular acidification and subsequent Na+/H+ exchanger activation contribute to incomplete RVD in Ehrlich ascites tumor cells.
  • The Na+/H+ exchanger plays a significant role in limiting the extent of regulatory volume decrease.
  • These findings suggest that intracellular acidification and Na+/H+ exchanger activation may be a general mechanism in cells undergoing RVD.