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Updated: Jun 1, 2026
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
Evaluation of oxidative stress status in children with pervasive developmental disorder and attention deficit
Insights
Children with pervasive developmental disorder (PDD) and attention deficit hyperactivity disorder (ADHD) show higher levels of oxidative stress biomarkers in urine. This non-invasive measurement could aid future research into these neurodevelopmental disorders.
Area of Science:
- Biochemistry
- Pediatrics
- Neuroscience
Background:
- Pervasive developmental disorder (PDD) and attention deficit hyperactivity disorder (ADHD) are neurodevelopmental conditions potentially linked to elevated oxidative stress.
- Oxidative stress, particularly lipid peroxidation, is implicated in the pathophysiology of PDD and ADHD.
Discussion:
- Urinary acrolein-lysine levels were significantly higher in pediatric patients with PDD and ADHD compared to control groups.
- Acrolein-lysine serves as a specific biomarker for lipid peroxidation, indicating increased oxidative stress.
- The non-invasive nature of urine sample analysis makes it a practical method for assessing oxidative stress in children.
Key Insights:
- Pediatric PDD and ADHD patients exhibit elevated urinary acrolein-lysine levels, suggesting increased lipid peroxidation.
- Urinary biomarker measurement offers a comfortable and non-invasive approach for evaluating oxidative stress in pediatric populations.
- Findings support the association between oxidative stress and neurodevelopmental disorders like PDD and ADHD.
Outlook:
- This study provides a foundation for further clinical and biochemical investigations into the role of oxidative stress in PDD and ADHD.
- The use of urinary biomarkers could facilitate larger-scale studies and potentially inform therapeutic strategies.
- Further research is warranted to explore the causal relationship and therapeutic interventions targeting oxidative stress in these disorders.
Abstract:
Pervasive developmental disorder (PDD) and attention deficit hyperactivity disorder (ADHD) are likely to be associated with increased oxidative stress, particularly that of lipid peroxidation. We evaluated the oxidative stress status of pediatric PDD and ADHD patients using their urine samples. Urinary acrolein-lysine levels in 11 PDD and 10 ADHD children (205 ± 97 and 234 ± 75 nmol/mg Cr, respectively) appeared higher than those of the control subjects (155 ± 59 nmol/mg Cr). Measurement of urinary specific biomarkers is comfortable, non-invasive, and easy to perform in children. Our findings might provide a scientific guide for use in further clinical and biochemical studies of these disorders.
