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Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: Jun 1, 2026

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

ES cell-derived renewable and functional midbrain dopaminergic progenitors.

Sangmi Chung1, Jung-Il Moon, Amanda Leung

  • 1Molecular Neurobiology Laboratory and Department of Psychiatry and Program in Neuroscience, Harvard Stem Cell Institute, McLean Hospital/Harvard Medical School, Belmont, MA 02478, USA. schung@mclean.harvard.edu

Proceedings of the National Academy of Sciences of the United States of America
|May 25, 2011
PubMed
Summary

Researchers developed a new method to isolate midbrain dopaminergic (mDA) neuronal progenitors (NPs) from stem cells. These expandable mDA NPs can differentiate into functional neurons, showing promise for Parkinson's disease treatments.

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Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
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Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Published on: September 15, 2014

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Last Updated: Jun 1, 2026

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
06:40

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Published on: September 15, 2014

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Midbrain dopaminergic (mDA) neuronal progenitors (NPs) are crucial for developing mature mDA neurons in the substantia nigra and ventral tegmental area.
  • Understanding the precise mechanisms and markers for isolating these progenitors is key for regenerative medicine applications.

Purpose of the Study:

  • To develop an efficient method for isolating authentic mDA NPs from differentiated embryonic stem cells (ESCs).
  • To characterize the proliferative capacity, differentiation potential, and in vivo functionality of these isolated mDA NPs.

Main Methods:

  • Utilized fluorescence-activated cell sorting (FACS) with Otx2 and Corin markers to isolate mDA NPs from differentiated ESCs.
  • Cultured and expanded isolated mDA NPs under optimized conditions.
  • Differentiated mDA NPs and assessed mDA neuronal marker expression and function.
  • Transplanted differentiated mDA NPs into Parkinson's disease (PD) model animals (aphakia mice and 6-OHDA-lesioned rats).

Main Results:

  • Efficient isolation of Otx2(+)Corin(+) mDA NPs expressing key progenitor markers.
  • Prolonged proliferation (up to 4 weeks, ~1,000-fold expansion) with stable phenotype.
  • Successful differentiation into functional mDA neurons with high purity.
  • In vivo transplantation led to integrated DA grafts, improved motor function in PD models, and significant cell migration without tumor formation.

Conclusions:

  • A novel FACS strategy efficiently isolates functional and expandable mDA NPs from ESCs.
  • These isolated mDA NPs hold significant potential for regenerative medicine, bioassays, and drug screening for neurodegenerative diseases like Parkinson's.