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Updated: Jun 1, 2026

Biocontained Carcass Composting for Control of Infectious Disease Outbreak in Livestock
Published on: May 6, 2010
Effects of continuous thermophilic composting (CTC) on bacterial community in the active composting process
Yong Xiao1, Guang-Ming Zeng, Zhao-Hui Yang
1College of Environmental Science and Engineering, Hunan University, Changsha 410082, China. xiaoyong1982@msn.com
Abstract:
The method of continuous thermophilic composting (CTC) remarkably shortened the active composting cycle and enhanced the compost stability. Effects of CTC on the quantities of bacteria, with a comparison to the traditional composting (TC) method, were explored by plate count with incubation at 30, 40 and 50°C, respectively, and by quantitative PCR targeting the universal bacterial 16S rRNA genes and the Bacillus 16S rRNA genes. The comparison of cultivatable or uncultivatable bacterial numbers indicated that CTC might have increased the biomass of bacteria, especially Bacillus spp., during the composting. Denaturing gradient gel electrophoresis (DGGE) analysis was employed to investigate the effects of CTC on bacterial diversity, and a community dominated by fewer species was detected in a typical CTC run. The analysis of sequence and phylogeny based on DGGE indicated that the continuously high temperature had changed the structure of bacterial community and strengthened the mainstay role of the thermophilic and spore-forming Bacillus spp. in CTC run.
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