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Updated: Jun 1, 2026

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Published on: December 27, 2016
(31)P NMR correlation maps of (18)O/ (16)O chemical shift isotopic effects for phosphometabolite labeling studies
Nenad Juranić1, Emirhan Nemutlu, Song Zhang
1Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, 55905, USA. juranic.nenad@mayo.edu
Abstract:
Intramolecular correlations among the (18)O-labels of metabolic oligophosphates, mapped by J-decoupled (31)P NMR 2D chemical shift correlation spectroscopy, impart stringent constraints to the (18)O-isotope distributions over the whole oligophosphate moiety. The multiple deduced correlations of isotopic labels enable determination of site-specific fractional isotope enrichments and unravel the isotopologue statistics. This approach ensures accurate determination of (18)O-labeling rates of phosphometabolites, critical in biochemical energy conversion and metabolic flux transmission. The biological usefulness of the J-decoupled (31)P NMR 2D chemical shift correlation maps was validated on adenosine tri-phosphate fractionally (18)O labeled in perfused mammalian hearts.
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