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Development of microsatellite markers in Kmeria septentrionalis (Magnoliaceae), an endangered Chinese tree
Yanfang Lin1, Liyan Zeng, Qiwei Zhang
1Ministry of Education Key Laboratory for Ecology of Rare and Endangered Species and Environmental Protection, School of Life Sciences, Guangxi Normal University, Guilin 541004, China.
Premise Of The Study:
Microsatellite markers were developed in an endangered plant, Kmeria septentrionalis, to investigate its population genetic structure, gene flow, and mating systems.
Methods And Results:
Using the combined biotin capture method, 14 microsatellite primer sets were isolated and characterized. All of these markers showed polymorphism, and the number of alleles per locus ranged from 3 to 15 across 60 individuals from two populations. The observed and expected heterozygosities ranged from 0.2692 to 0.9667 and from 0.3271 to 0.8881, respectively.
Conclusions:
These markers will facilitate further studies on the genetic diversity and mating systems of K. septentrionalis.
