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Published on: August 5, 2020
Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)
Michelle Waycott1, Brendan L Jones, Jent Kornelis Van Dijk
1School of Marine and Tropical Biology, James Cook University, Townsville, QLD 4811, Australia.
Premise Of The Study:
The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified.
Methods And Results:
Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14.
Conclusions:
These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.
