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Published on: February 28, 2015
Development of new microsatellite markers from Mango (Mangifera indica) and cross-species amplification
Kundapura Venkataramana Ravishankar1, Bellam Hanumantha-Reddy Mani, Lalitha Anand
1Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post,Bangalore 560 089, India. kv_ravishankar@yahoo.co.in
Premise Of The Study:
Microsatellite markers were developed and characterized to assess the genetic diversity among mango (Mangifera indica) cultivars and to test their amplification in closely related species.
Methods And Results:
Thirty-six microsatellite (simple sequence repeats; SSR) loci were isolated by a microsatellite-enriched partial genomic library method. Primers designed for these loci were characterized using 30 diverse mango cultivars. The number of alleles ranged from 3 to 19 with an average of 9.2 alleles per locus. Polymorphic information content values ranged from 0.185 to 0.920 with a mean of 0.687. The total value for the probability of identity was 2.42 × 10(-31).
Conclusions:
The newly identified SSRs would be useful in genetic diversity studies, finger-printing, and mapping. Loci from five related species, M. odorata, M. anadamanica, M. zeylanica, M. camptosperma, and M. griffithii, were successfully amplified using these SSR primers, showing their potential utility across species.
