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Related Concept Videos

Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Crossing Over01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...
Crossing Over01:34

Crossing Over

Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...

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Related Experiment Video

Updated: Jun 1, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Defined-size DNA triple crossover construct for molecular electronics: modification, positioning and conductance

Veikko Linko1, Jenni Leppiniemi, Seppo-Tapio Paasonen

  • 1Nanoscience Center, Department of Physics, University of Jyväskylä, PO Box 35, FIN-40014, Finland. veikko.linko@jyu.fi

Nanotechnology
|May 27, 2011
PubMed
Summary

We developed a novel DNA structure (B-A-B complex) for precise molecular patterning in nanoelectronics. This rigid, defined-size template enables advanced applications in plasmonics and sensing, showcasing its potential for nanoscale engineering.

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Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Materials Science

Background:

  • DNA nanotechnology offers precise control over molecular assembly.
  • Developing stable, defined-size DNA structures is crucial for nanoscale applications.
  • Existing DNA structures may lack the rigidity or defined size required for certain applications.

Purpose of the Study:

  • To introduce a novel, defined-size, small, and rigid DNA template called a B-A-B complex.
  • To demonstrate the feasibility of using B-A-B complexes for molecular scale patterning.
  • To characterize the electrical conductance properties of the B-A-B complex.

Main Methods:

  • DNA triple crossover (TX) motifs were used to construct the B-A-B complex.
  • TX tiles were functionalized with biotin-triethylene glycol (TEG) and decorated with streptavidin.
  • Single thiol-modified B-A-B complexes were positioned and anchored to a chip via dielectrophoretic trapping.
  • DC conductivity measurements and AC impedance spectroscopy were employed to analyze conductance properties.

Main Results:

  • The B-A-B complex was successfully synthesized and functionalized.
  • Single B-A-B complexes were precisely positioned and anchored on a chip.
  • The conductance properties of the non-functionalized B-A-B complex were characterized.
  • An equivalent circuit model described the conductivity mechanism of a single B-A-B complex.

Conclusions:

  • The B-A-B complex is a viable DNA template for molecular scale patterning.
  • The study provides insights into the conductivity mechanisms of DNA structures.
  • This work paves the way for advanced nanoelectronic, plasmonic, and sensing applications using DNA.