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Published on: September 13, 2022
Screening and initial binding assessment of fumonisin b(1) aptamers
Maureen McKeague1, Charlotte R Bradley, Annalisa De Girolamo
1Chemistry Department, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada K1S 5B6; E-Mails: mmckeagu@connect.carleton.ca (M.M.); cbradle4@connect.carleton.ca (C.R.B.); david_miller@carleton.ca (J.D.M.).
Abstract:
Fumonisins are mycotoxins produced by Fusarium verticillioides and F. proliferatum, fungi that are ubiquitous in corn (maize). Insect damage and some other environmental conditions result in the accumulation of fumonisins in corn-based products worldwide. Current methods of fumonisin detection rely on the use of immunoaffinity columns and high-performance liquid chromatography (HPLC). The use of aptamers offers a good alternative to the use of antibodies in fumonisin cleanup and detection due to lower costs and improved stability. Aptamers are single-stranded oligonucleotides that are selected using Systematic Evolution of Ligands by EXponential enrichment (SELEX) for their ability to bind to targets with high affinity and specificity. Sequences obtained after 18 rounds of SELEX were screened for their ability to bind to fumonisin B(1). Six unique sequences were obtained, each showing improved binding to fumonisin B(1) compared to controls. Sequence FB(1) 39 binds to fumonisin with a dissociation constant of 100 ± 30 nM and shows potential for use in fumonisin biosensors and solid phase extraction columns.
