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Published on: October 22, 2018
Fe-chlorophyllin promotes the growth of wheat roots associated with nitric oxide generation
Min Tong1, Liefeng Zhang, Yifan Wang
1Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing 210046, China; E-Mails: tongmin@163.com (M.T.); yifan@gmail.com (Y.W.); Jianghui523@163.com (H.J.).
Abstract:
Effects of Fe-chlorophyllin on the growth of wheat root were investigated in this study. We found that Fe-chlorophyllin can promote root growth. The production of nitric oxide in wheat root was detected using DAF-2DA fluorescent emission. The intensity of fluorescent in the presence of 0.1 mg/L Fe-chlorophyllin was near to that observed with the positive control of sodium nitroprusside (SNP), the nitric oxide donor. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control. Meanwhile, Fe-chlorophyllin treatment could increase the activities of reactive oxygen scavenging enzymes, such as superoxide dismutase (SOD) and peroxidase (POD), as determined using non-denaturing polyacrylamide gel electrophoresis. These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.
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