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Updated: Jun 1, 2026

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries
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A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

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Microfabricated polyester conical microwells for cell culture applications.

Seila Selimović1, Francesco Piraino, Hojae Bae

  • 1Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA.

Lab on a Chip
|May 27, 2011
PubMed
Summary

Researchers developed a fast, low-cost laser ablation method to create microwells on polyester films for cell culture. This technique enables efficient high-throughput screening of cell behavior in stem cell research and other applications.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Lab-on-a-chip systems are gaining interest for high-throughput screening, particularly in stem cell research.
  • Microwells are preferred structures for these microscale devices.
  • Existing microwell fabrication methods (photolithography, etching) are often equipment-intensive, time-consuming, and costly to modify.

Purpose of the Study:

  • To develop a convenient, fast, and low-cost method for fabricating microwells for cell culture applications.
  • To demonstrate the utility of laser ablation for creating customizable microwell platforms.
  • To assess the suitability of the fabricated microwells for cell culture and aggregate formation.

Main Methods:

  • Laser ablation of polyester film coated with silicone glue.

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Last Updated: Jun 1, 2026

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries
11:42

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

Published on: January 28, 2018

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

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Microfabricated Platforms for Mechanically Dynamic Cell Culture

Published on: December 26, 2010

  • Control of microwell diameter via laser power and speed.
  • Control of microwell depth by stacking multiple film layers.
  • Seeding of murine embryonic stem cells and human hepatoblastoma cells in fabricated microwells.

    • Main Results:

    • A simple, rapid, and inexpensive method for microwell fabrication was established.
    • Microwell dimensions (diameter and depth) were controllable.
    • Hundreds of microwells could be fabricated within minutes.
    • Cell aggregates formed in polyester microwells within 9 days without apparent negative effects from the materials.

    Conclusions:

    • The developed laser ablation technique offers a user-friendly and cost-effective approach for microwell fabrication.
    • The polyester microwell platform is suitable for cell culture applications, including the formation of cell aggregates.
    • This method provides a valuable tool for cell behavior analysis and high-throughput screening in microscale devices.