Ten polymorphic microsatellite markers in Michelia maudiae (Magnoliaceae)
Ye Sun1, Yi-Fei Liu, Jing Wang
1Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China.
Premise Of The Study:
Michelia maudiae is a threatened species in the Magnoliaceae. Microsatellite markers were developed and characterized in M. maudiae for further investigation of its conservation genetics. •
Methods And Results:
Microsatellite markers were developed in M. maudiae using the Fast Isolation by AFLP of Sequences Containing Repeats protocol. Ten polymorphic microsatellites were assessed in two populations of M. maudiae. The number of alleles detected per locus varied from 1 to 8. Observed and expected heterozygosities ranged from 0.000 to 0.792 and from 0.000 to 0.826, respectively. Six primer pairs showed transferability in the two related species Michelia foveolata and Michelia chapensis. •
Conclusions:
This set of microsatellite markers provides a useful tool for future studies of the conservation genetics of M. maudiae and other congeneric species.
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