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Updated: Jun 1, 2026

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

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New microsatellite loci for pomegranate, Punica granatum (Lythraceae)

Sergio Currò¹, Marco Caruso, Gaetano Distefano

¹Dipartimento di OrtoFloroArboricoltura e Tecnologie Agroalimentari, University of Catania, Via Valdisavoia 5 Catania 95123 Italy.

American Journal of Botany

|May 28, 2011

Abstract

Premise Of The Study:

A new set of pomegranate microsatellites was selected and characterized to assess the level of genetic diversity among cultivars and wild genotypes. •

Methods And Results:

Nine Simple Sequence Repeat (SSR) markers were obtained using the Microsatellite-AFLP technique and were successfully amplified in 34 genotypes belonging to Italian, Spanish, and Turkish germplasm collections. The number of alleles per locus ranged from 1 to 5, and the total number of alleles was 22. •

Conclusions:

Because only a few codominant markers are available for this species, the newly identified SSRs will facilitate genetic diversity studies, fingerprinting, and mapping. In addition, the 9 loci successfully amplified in P. granatum var. nana. No cross transferability was observed for Cuphea micropetala and Lagerstroemia indica (Lythraceae).

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