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Related Experiment Video

Updated: Jun 1, 2026

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Published on: May 6, 2013

IL-13Rα1 expression on β-cell-specific T cells in NOD mice.

Sarah S Rasche¹, Michele Phillips, Marcia F McInerney

¹Department of Biological Sciences, University of Toledo, Toledo, Ohio, USA.

|May 28, 2011

Summary

Interleukin-13 (IL-13) plays a key role in regulating early-stage insulitis in diabetes-prone mice. Loss of IL-13 receptor α1 (IL-13Rα1) on T cells may indicate immune dysregulation and disease progression.

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Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices

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Published on: April 12, 2021

Area of Science:

Immunology
Endocrinology
Autoimmunity

Background:

Immunotherapy with GAD65 peptides can preserve glucose homeostasis in non-obese diabetic (NOD) mice.
Previous studies identified GAD65 epitopes 524-538 and 530-543, with 524-538 linked to reduced hyperglycemia.

Purpose of the Study:

To investigate if T cells specific for peptide p524-538 can regulate islet-reactive T cells.
To determine the role of IL-13 and IL-13Rα1 in the regulation of islet autoimmunity in NOD mice.

Main Methods:

Prediabetic NOD mice were used to study the relationship between peptide p524-538-induced IL-13 and islet autoimmunity.
Pancreatic lymph node (PLN) cells were analyzed for IL-13 receptor α1 (IL-13Rα1) expression on T cells at different stages of insulitis.

Main Results:

Peptide p524-538 induced IL-13-producing T cells that counteracted GAD65 autoimmunity and inhibited islet-reactive T cell proliferation.
Functional IL-13Rα1 was expressed on a subset of CD4(+) T cells, and its expression was higher in young NOD mice compared to older mice with advanced insulitis.
Immunization with p524-538 maintained IL-13Rα1 expression on PLN T cells.

Conclusions:

IL-13 is crucial for regulating autoimmunity during early insulitis stages.
Loss of IL-13Rα1 on islet-reactive T cells may serve as a biomarker for diminished immune regulation and progression to type 1 diabetes.