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Related Experiment Video

Updated: Jun 1, 2026

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
09:52

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

A V-shaped cationic dye for nonlinear optical bioimaging.

Ermelinda Maçôas1, Gema Marcelo, Sandra Pinto

  • 1Centro de Química-Física Molecular (CQFM) and Institute of Nanoscience and Nanotechnology (IN), Instituto Superior Técnico, 1049-001 Lisboa, Portugal. ermelinda.macoas@ist.utl.pt

Chemical Communications (Cambridge, England)
|May 28, 2011
PubMed
Summary

A novel symmetric cationic molecule was synthesized for advanced cellular imaging. This fluorescent marker effectively stains organelles and penetrates the nucleus in living cells.

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Area of Science:

  • Materials Science
  • Biophysics
  • Cell Biology

Background:

  • Developing advanced fluorescent probes is crucial for high-resolution cellular imaging.
  • Molecules with D-π-A(+)-π-D architecture offer unique photophysical properties.

Purpose of the Study:

  • To synthesize and characterize a novel symmetric cationic molecule for fluorescence microscopy.
  • To evaluate its performance as a cellular marker in living cells.

Main Methods:

  • Synthesis of a D-π-A(+)-π-D symmetric cationic molecule.
  • Two-photon absorption cross-section (σ(2)) measurement.
  • Fluorescence microscopy and fluorescence lifetime imaging microscopy (FLIM) in living cells.

Main Results:

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Last Updated: Jun 1, 2026

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
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Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

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  • The synthesized molecule exhibited a high two-photon absorption cross-section (σ(2) ≈ 1140 GM).
  • The probe successfully localized within living cells, staining vesicular organelles in the cytoplasm.
  • FLIM demonstrated the probe's ability to penetrate the cell nucleus.

    • Conclusions:

    • The novel symmetric cationic molecule is a promising fluorescent probe for live-cell imaging.
    • Its D-π-A(+)-π-D architecture facilitates efficient two-photon absorption and cellular uptake.
    • The probe's dual localization in cytoplasm and nucleus enhances its utility in cell biology research.