Related Experiment Video

Updated: Jun 1, 2026

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Tissue factor is involved in retinoblastoma cell proliferation via both the Akt and extracellular signal-regulated

Byung Joo Lee1, Jeong Hun Kim, Seung Hoon Woo

  • 1Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, and Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea.

Oncology Reports
|May 28, 2011
PubMed

Insights

Tissue factor (TF) drives retinoblastoma cell proliferation. Blocking the TF pathway with TF pathway inhibitor (TFPI) suppressed tumor growth and key signaling pathways, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Ophthalmology

Background:

  • Tissue factor (TF) is implicated in tumor progression.
  • The role and expression of TF in retinoblastoma remain uncharacterized.

Purpose of the Study:

  • To investigate the expression and function of TF in retinoblastoma.
  • To determine if TF pathway blockade can inhibit retinoblastoma cell proliferation.

Main Methods:

  • TF expression analysis in an orthotopic retinoblastoma mouse model.
  • Assessment of TF pathway inhibitor (TFPI) effects on FGF2-stimulated retinoblastoma cells.
  • Evaluation of Akt and ERK1/2 phosphorylation levels.

Main Results:

  • TF is selectively expressed in highly proliferative areas of retinoblastoma tumors.
  • FGF2 treatment increased TF expression and retinoblastoma cell proliferation.
  • TFPI significantly inhibited FGF2-induced proliferation and phosphorylation of Akt and ERK1/2.

Conclusions:

  • TF expression is closely linked to retinoblastoma cell proliferation.
  • TFPI demonstrates potential as a therapeutic agent by inhibiting retinoblastoma growth via Akt and ERK1/2 pathways.

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