Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Hematopoiesis

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The KDM-family inhibitor JIB-04 sensitizes AML cells to venetoclax by inducing a ferroptosis-like phenotype.

Blood neoplasia·2026

Same author

Polθ activity modulates sensitivity to standard therapies in DNMT3A-deficient leukemia.

Cell reports. Medicine·2026

Same author

Age-associated hematopoietic variants in the rhesus macaque.

Blood advances·2026

Same author

Comparison with Ezh2 reveals the PRC2-dependent functions of Jarid2 in hematopoietic stem Cell lineage commitment.

Stem cell reports·2026

Same author

Disparate leukemia mutations converge on nuclear phase-separated condensates.

Cell·2025

Same author

Cohesin haploinsufficiency is tolerated in Cbfb::MYH11-driven murine acute myeloid leukemia.

Experimental hematology·2025

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

Hematopoietic stem cell characterization and isolation.

Lara Rossi¹, Grant A Challen, Olga Sirin

¹Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 28, 2011

Summary

This summary is machine-generated.

Hematopoietic stem cells (HSCs) are crucial for blood system regeneration and can be purified using their unique antigenic (KSL) and dye efflux (Side Population) properties. This method aids in isolating these rare cells for research and potential therapies.

More Related Videos

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Published on: May 19, 2023

Stromal Cell Isolation From Hematopoietic Organs

Stromal Cell Isolation From Hematopoietic Organs

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Jun 1, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Published on: May 19, 2023

Stromal Cell Isolation From Hematopoietic Organs

Stromal Cell Isolation From Hematopoietic Organs

Published on: January 26, 2024

Area of Science:

Hematology
Stem Cell Biology
Cellular Immunology

Background:

Hematopoietic stem cells (HSCs) possess self-renewal and differentiation capabilities essential for maintaining blood homeostasis.
HSCs are critical for restoring hematopoiesis after damage and rely on chemokine-guided migration for homing to the bone marrow.
HSCs are a rare cell population (0.01% of nucleated cells) residing in the bone marrow, posing a challenge for isolation.

Purpose of the Study:

To detail a laboratory strategy for purifying murine HSCs.
To leverage specific antigenic markers and dye efflux properties for HSC isolation.

Main Methods:

Utilizing the KSL (c-Kit+, Sca-1+, lineage-) antigenic phenotype to identify HSCs.
Exploiting the Side Population (SP) characteristic, which represents cells efficiently effluxing the vital dye Hoechst 33342.
Combining KSL and SP properties for enhanced purification of murine HSCs.

Main Results:

The described method allows for the purification of HSCs based on their unique biological properties.
The KSL phenotype combined with the SP characteristic effectively isolates HSCs from other bone marrow cells.

Conclusions:

The KSL and SP method provides a robust strategy for isolating murine HSCs.
This purification technique is vital for studying HSC function and developing regenerative medicine approaches.