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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

miRvar: A comprehensive database for genomic variations in microRNAs.

Deeksha Bhartiya1, Saurabh V Laddha, Arijit Mukhopadhyay

  • 1GN Ramachandran Knowledge Center for Genome Informatics, Institute of Genomics and Integrative Biology (CSIR), Mall Road, Delhi 110007.

Human Mutation
|May 28, 2011
PubMed
Summary

This study systematically curates genetic variations in microRNA (miRNA) loci, establishing a computational pipeline to analyze their functional consequences. The comprehensive dataset is now available on the Leiden Open Variation Database for broader research and community input.

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) are key regulators in biological processes.
  • Previous research has not systematically analyzed genetic variations within miRNA loci and their functional impacts.

Purpose of the Study:

  • To comprehensively curate genetic variations in human miRNA loci.
  • To develop a computational pipeline for assessing the functional consequences of these variants.
  • To establish systematic methods for variation curation and reporting.

Main Methods:

  • Systematic curation of genetic variations in miRNA loci.
  • Development of a computational pipeline for functional consequence assessment.
  • Data integration and reporting on the Leiden Open Variation Database (LOVD).

Main Results:

  • A comprehensive dataset of genetic variations in miRNA loci has been curated.
  • A functional assessment pipeline for miRNA variants is established.
  • The curated data is accessible via the http://genome.igib.res.in/mirlovd platform.

Conclusions:

  • This work provides a foundational resource for understanding genetic variation in miRNAs.
  • The developed pipeline and database facilitate the analysis of miRNA variant functions.
  • The open-access platform encourages community participation in miRNA variation research.