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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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Related Experiment Video

Updated: Jun 1, 2026

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

Unraveling DNA dynamics using atomic force microscopy.

Yuki Suzuki1, Yuko Yoshikawa, Shige H Yoshimura

  • 1Laboratory of Plasma Membrane and Nuclear Signaling, Graduate School of Biostudies, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, Japan. ysuzuki79.m07@lif.kyoto-u.ac.jp.

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
|May 28, 2011
PubMed
Summary
This summary is machine-generated.

Atomic Force Microscopy (AFM) reveals crucial DNA properties and dynamics, aiding understanding of gene regulation and molecular mechanisms. This review highlights AFM

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Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

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Area of Science:

  • Biophysics
  • Molecular Biology
  • Genomics

Background:

  • Understanding DNA structure-function relationships is vital in post-genomic research.
  • Elucidating molecular mechanisms of gene regulation requires knowledge of DNA properties and dynamics.
  • Atomic Force Microscopy (AFM) offers topographical and physicochemical insights into DNA and DNA-protein complexes.

Purpose of the Study:

  • To review the application of AFM techniques in studying DNA and DNA-protein complexes.
  • To explore how AFM analyses advance the understanding of DNA dynamics.
  • To discuss AFM's role in investigating DNA's fundamental features and interactions.

Main Methods:

  • Utilizing Atomic Force Microscopy (AFM) to probe DNA topological transitions, length-dependent properties, and flexibility.
  • Employing advanced AFM with high time-resolution and improved sample preparation for analyzing DNA-protein interactions.
  • Investigating DNA tension-dependent enzymatic reactions and DNA looping dynamics at the nanoscale.

Main Results:

  • AFM reveals fundamental DNA features like topological transitions, length-dependent properties, and non-Watson-Crick base pairing.
  • High-resolution AFM enables visualization of DNA-protein interactions in real-time.
  • Physical properties of DNA influence nucleosome dynamics and chromatin higher-order structure transitions.

Conclusions:

  • AFM is a powerful tool for dissecting DNA dynamics and structure-function relationships.
  • AFM advancements facilitate nanoscale investigations of DNA-protein interactions and enzymatic activities.
  • Understanding DNA's physical properties is key to comprehending gene regulation and chromatin organization.