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Related Concept Videos

Disorders of Leukocytes01:27

Disorders of Leukocytes

Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
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An adult in good health typically has between 4,500 and 11,000 leukocytes, or white blood cells, per microliter of blood, which constitutes about 1% of the total blood volume. Unlike red blood cells, white blood cells contain a nucleus and other cellular organelles but do not have hemoglobin. Most white blood cells reside in connective tissues, particularly in lymphatic organs such as the lymph nodes, with only a small fraction present in circulating blood.
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Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors
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Published on: January 7, 2019

CD97 in leukocyte trafficking.

Jörg Hamann1, Henrike Veninga, Dorien M de Groot

  • 1Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. j.hamann@amc.uva.nl

Advances in Experimental Medicine and Biology
|May 31, 2011
PubMed
Summary
This summary is machine-generated.

Targeting CD97 with monoclonal antibodies inhibits innate immune cell recruitment, impacting inflammation and host defense. This approach shows therapeutic potential in models like rheumatoid arthritis but not adaptive immunity.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Medicine

Background:

  • CD97, an adhesion G protein-coupled receptor (GPCR) on leukocytes, interacts with ligands via its EGF-like domains.
  • Understanding CD97's function is crucial for immune response modulation.

Purpose of the Study:

  • To investigate the biological function of CD97 using monoclonal antibodies (mAbs) in various mouse models of innate and adaptive immunity.
  • To explore the therapeutic potential of targeting CD97.

Main Methods:

  • Utilized monoclonal antibodies (mAbs) targeting specific EGF domains of CD97.
  • Employed in vivo mouse models including antibacterial defense, inflammatory disorders, stem cell mobilization, delayed type hypersensitivity (DTH), experimental autoimmune encephalomyelitis (EAE), and collagen-induced arthritis (CIA).
  • Compared effects of antibody treatment with CD97 gene targeting.

Main Results:

  • CD97 targeting by mAbs inhibited neutrophilic granulocyte accumulation at inflammatory sites, affecting antibacterial defense and stem cell mobilization.
  • No impact was observed on adaptive immune responses (DTH, EAE).
  • Significant amelioration of collagen-induced arthritis (CIA) suggests therapeutic value.

Conclusions:

  • CD97 mAbs actively inhibit innate immune responses, likely by blocking granulocyte and macrophage recruitment.
  • Proposed mechanisms include activating CD97 signaling or inducing receptor internalization, hindering ligand binding.
  • CD97 targeting holds therapeutic promise for inflammatory conditions like rheumatoid arthritis.