Related Experiment Video
Updated: Jun 1, 2026

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
Published on: January 30, 2019
Detection and confirmation of N-nitrosodialkylamines using liquid chromatography-electrospray ionization coupled
D A Volmer1, J O Lay, S M Billedeau
1National Center for Toxicological Research, 3900 NCTR Road, Jefferson, Arkansas 72079.
Abstract:
Simultaneous detection and confirmation of several N-nitrosodialkylamines are accomplished by on-line coupling of a photolysis reactor with an HPLC-electrospray ionization mass spectrometer. Several parameters such as irradiation wavelength, irradiation time, mobile-phase composition, and pH, as well as different organic acid modifiers are investigated, and their impact on the detection of the N-nitrosodialkylamine-acid complex and its dissociative photolysis products is presented here. Additionally, the type of structural information obtained from the photolytic processes of N-nitrosodialkylamines is compared to that obtained by using in-source collision-induced dissociation. To demonstrate the potential of this technique, six N-nitrosodialkylamines are studied to determine the linearity of the response, the limits of detection and confirmation, and the reproducibility. The technique's versatility is also exhibited by utilizing negative-ion mode as a complementary means for analysis of the compounds. Finally, an illustrative application for N-nitrosodimethylamine analysis in beer is described.
More Related Videos
07:38A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
08:41Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
Related Concept Videos
Gas Chromatography: Types of Detectors-II
High-Performance Liquid Chromatography: Types of Detectors
2° Amines to N-Nitrosamines: Reaction with NaNO2
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Mass Spectrometry of Amines