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Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)

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Macromolecule analysis based on electrophoretic mobility in air: globular proteins.

S L Kaufman¹, J W Skogen, F D Dorman

¹TSI Incorporated, 500 Cardigan Road, St. Paul, Minnesota 55126.

Analytical Chemistry

|May 31, 2011

Summary

This study presents an aerosol-based method for analyzing globular proteins by measuring their electrophoretic mobility. The technique effectively separates proteins by size, offering a novel approach for protein characterization.

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Area of Science:

Analytical Chemistry
Biophysics
Aerosol Science

Background:

Characterizing globular proteins is crucial for understanding biological functions.
Existing methods for protein analysis can be complex and time-consuming.
Aerosol-based techniques offer potential for rapid, sensitive measurements.

Purpose of the Study:

To develop and validate an aerosol technique for separating and analyzing globular proteins.
To determine the feasibility of using electrophoretic mobility in air for protein mass determination.
To establish a method for characterizing proteins across a wide molecular mass range.

Main Methods:

Proteins were analyzed using an aerosol technique based on electrophoretic mobility of singly-charged molecular ions in air.
Ions were generated via electrospraying and drying of protein suspensions.
Electrophoretic mobility was measured using a modified differential mobility analyzer near atmospheric pressure.

Main Results:

The technique successfully separated globular proteins with molecular masses from 5.7 to 669 kDa.
Results were expressed as electrophoretic mobility equivalent diameters using the Millikan formula.
Analyzed protein concentrations ranged from 0.02 to 200 μg/mL with a 3-minute sampling time.

Conclusions:

The developed aerosol technique provides an effective means for separating and analyzing globular proteins.
This method offers a novel approach for rapid protein characterization based on size.
The technique demonstrates potential for broader applications in biophysical and biochemical analyses.