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Rate-limiting steps of tyrosinase-modified electrodes for the detection of catechol

E Burestedt1, A Narvaez, T Ruzgas

  • 1Department of Analytical Chemistry, University of Lund, Box 124, S-221 00 Lund, Sweden, Department of Analytical Chemistry, Faculty of Pharmacy, University of Alcalá de Henares, 28 871 Alcalá de Henares, Madrid, Spain, and Enzyme Chemistry Laboratory, Institute of Biochemistry, Mokslininku 12, 2600 Vilnius, Lithuania.

Analytical Chemistry
|May 31, 2011
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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...