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A nitrite sensor based on a highly sensitive nitrite reductase mediator-coupled amperometric detection.

B Strehlitz1, B Gründig, W Schumacher

  • 1Umweltforschungszentrum Leipzig-Halle GmbH, Permoserstrasse 15, D 04318 Leipzig, Germany, SensLab GmbH, Leonhard-Frank-Strasse 7, D 04318 Leipzig, Germany, Fakultät für Biologie, Universität Konstanz, Universitätsstrasse 10, D 78434 Konstanz, Germany, and Bundesforschungsanstalt für Landwirtschaft, Institut für Technologie, Bundesallee 50, D38116 Braunschweig, Germany.

Analytical Chemistry
|May 31, 2011
PubMed
Summary

Highly sensitive nitrite sensors were developed using mediator-modified electrodes and enzymes. These novel sensors offer precise nitrite detection with a low detection limit, paving the way for advanced analytical applications.

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Published on: December 25, 2016

Area of Science:

  • Electrochemistry
  • Biotechnology
  • Analytical Chemistry

Background:

  • Development of highly sensitive nitrite sensors is crucial for various applications.
  • Enzyme-based electrochemical sensors offer high specificity and sensitivity.
  • Mediator-modified electrodes enhance electron transfer between enzymes and electrodes.

Purpose of the Study:

  • To develop novel, highly sensitive nitrite sensors using mediator-modified electrodes.
  • To investigate the performance of different electron mediators and enzymes for nitrite detection.
  • To characterize the electrochemical properties and optimize the sensor for nitrite quantification.

Main Methods:

  • Utilized tetraheme cytochrome c nitrite reductase and cytochrome cd(1) nitrite reductase.
  • Employed electron mediators including phenazines and triarylmethane redox dyes.
  • Immobilized enzymes in poly(carbamoyl sulfonate) (PCS) hydrogel on graphite electrodes.
  • Characterized sensors using chronoamperometry and cyclic voltammetry.

Main Results:

  • Phenosafranin-modified electrodes with PCS-immobilized tetraheme cytochrome c nitrite reductase showed linear current responses up to 250 μM nitrite.
  • Achieved a high sensitivity of 446.5 mA M(-1) cm(-2).
  • Established a low detection limit of 1 μM nitrite for the enzymatic sensor.

Conclusions:

  • Successfully developed highly sensitive nitrite sensors based on mediator-modified electrodes and immobilized enzymes.
  • Demonstrated the efficiency of phenosafranin as an electron mediator for nitrite detection.
  • The developed sensor shows significant potential for accurate and sensitive nitrite analysis.