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Updated: Jun 1, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
Published on: November 21, 2023
Integrated on-capillary electrochemical detector for capillary electrophoresis
1Departments of Chemistry and Pharmaceutical Chemistry and Center for Bioanalytical Research, University of Kansas, 2095 Constant Avenue, Lawrence, Kansas 66047.
Abstract:
An on-capillary electrochemical detector for capillary electrophoresis is described. It consists of a gold wire mounted permanently at the end of the capillary perpendicular to the direction of flow. This mode of detection eliminates the need for the micromanipulators or specially machined cell holders for alignment that are used for in-capillary detection modes. It also makes it possible to perform relatively fast CEEC separations using very short capillaries. The use of this detector for both off-column detection of catecholamines and end-column detection of carbohydrates by CE-PAD is described.
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