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Published on: December 4, 2021
Sample preparation using a miniaturized supported liquid membrane device connected on-line to packed capillary liquid
E Thordarson1, S Pálmarsdóttir, L Mathiasson
1Department of Analytical Chemistry, University of Lund, P.O. Box 124, 221 00 Lund, Sweden.
Analytical Chemistry
|May 31, 2011
Summary
A novel miniaturized supported liquid membrane device enables on-line sample preparation for liquid chromatography. This system efficiently enriches analytes, achieving high repeatability and low detection limits in plasma samples.
Area of Science:
- Analytical Chemistry
- Separation Science
- Biomedical Engineering
Background:
- Traditional sample preparation methods can be time-consuming and labor-intensive.
- Miniaturization of analytical devices is crucial for high-throughput and point-of-care applications.
- Supported liquid membranes offer selective analyte enrichment with low solvent consumption.
Purpose of the Study:
- To develop and validate a miniaturized supported liquid membrane (SLM) device for on-line sample preparation.
- To integrate the SLM device with a packed capillary liquid chromatograph (LC).
- To assess the performance of the SLM-LC system for analyte enrichment and detection in complex matrices.
Main Methods:
- Fabrication of a miniaturized SLM device using a hydrophobic polypropylene hollow fiber in a Kel-F holder.
- Impregnation of the hollow fiber pores with 6-undecanone to form the liquid membrane.
- On-line coupling of the SLM device to a packed capillary LC system.
- Testing with bambuterol as a model analyte in aqueous solutions and human plasma.
Main Results:
- The SLM device achieved selective enrichment of bambuterol with acceptor volumes as low as 1-2 μL.
- Optimal extraction efficiency of 32.5% was observed in aqueous solutions at a donor flow rate of 2.5 μL/min.
- A concentration enrichment factor of approximately 0.9 times/min was achieved at flow rates above 20 μL/min.
- Repeatability (RSD) of ~4% for spiked plasma samples and a detection limit of 80 nM for bambuterol in plasma were obtained.
Conclusions:
- The developed miniaturized SLM device is effective for on-line sample preparation coupled with capillary LC.
- The system demonstrates good enrichment capabilities, high repeatability, and low detection limits for analytes in biological samples.
- This approach offers a promising solution for efficient and sensitive analysis of pharmaceuticals and other compounds in complex matrices.
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