Graphite/Liquid mixed matrices for laser desorption/ionization mass spectrometry
M J Dale1, R Knochenmuss, R Zenobi
1Department of Chemistry, ETH Zentrum, CH-8092, Zürich, Switzerland.
Analytical Chemistry
|May 31, 2011
Summary
This study shows that combining a liquid matrix with graphite particles offers a versatile method for ultraviolet laser desorption/ionization mass spectrometry of diverse analytes like peptides and polymers.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Materials Science
Background:
- Laser desorption/ionization mass spectrometry (LD-MS) is crucial for analyzing biomolecules.
- Previous methods using liquid matrix and graphite particulates showed promise for peptide and protein analysis.
Purpose of the Study:
- To demonstrate the utility of liquid matrix and graphite particulates for ultraviolet laser desorption/ionization (UV-LD-MS).
- To investigate the mechanism, preparation, and application scope of this analytical approach.
Main Methods:
- Utilized a combination of liquid matrix and graphite particulates (2 μm) for UV-LD-MS.
- Investigated desorption/ionization mechanisms, preparative procedures, and analyte range.
- Analyzed peptides, proteins, oligosaccharides, and synthetic polymers.
Main Results:
- Achieved straightforward and general UV-LD-MS for a broad range of analytes.
- Demonstrated femtomole detection sensitivities for peptides and oligosaccharides.
- Graphite efficiently transfers UV energy for thermal desorption; liquid matrix aids protonation, cationization, and sample stability.
Conclusions:
- The liquid matrix-graphite particulate method is a robust and versatile technique for UV-LD-MS.
- This approach simplifies sample preparation and enhances analyte detection sensitivity.
- The method is applicable to a wide array of compounds, including synthetic polymers with added cationizing salts.
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