Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

Gas Chromatography–Mass Spectrometry (GC–MS)

Gas Chromatography–Mass Spectrometry (GC–MS)

Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...

Electrospray Ionization (ESI) Mass Spectrometry

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Breathprints of the Barn: The Future of Livestock Research and Monitoring with Exhalomics.

Annual review of animal biosciences·2025

Same author

Exhalomics as a noninvasive method for assessing rumen fermentation in dairy cows: Can exhaled-breath metabolomics replace rumen sampling?

Journal of dairy science·2023

Same author

Exhaled volatile fatty acids, ruminal methane emission, and their diurnal patterns in lactating dairy cows.

Journal of dairy science·2023

Same author

KFLC Index utility in multiple sclerosis diagnosis: Further confirmation.

Journal of neuroimmunology·2017

Same author

Author's response to Letter to the Editor by N. Messaoudani, R. Djidjik and M. Ghaffor published in JNI 266 (2014) entitled "Comments on CSF κFLC assay evaluation in assessing intrathecal synthesis".

Journal of neuroimmunology·2017

Same author

Gauging circadian variation in ketamine metabolism by real-time breath analysis.

Chemical communications (Cambridge, England)·2017

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Graphite/Liquid mixed matrices for laser desorption/ionization mass spectrometry.

M J Dale¹, R Knochenmuss, R Zenobi

¹Department of Chemistry, ETH Zentrum, CH-8092, Zürich, Switzerland.

Analytical Chemistry

|May 31, 2011

Summary

This study shows that combining a liquid matrix with graphite particles offers a versatile method for ultraviolet laser desorption/ionization mass spectrometry of diverse analytes like peptides and polymers.

More Related Videos

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Published on: July 29, 2018

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Published on: June 30, 2023

Related Experiment Videos

Last Updated: Jun 1, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Published on: July 29, 2018

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Published on: June 30, 2023

Area of Science:

Analytical Chemistry
Mass Spectrometry
Materials Science

Background:

Laser desorption/ionization mass spectrometry (LD-MS) is crucial for analyzing biomolecules.
Previous methods using liquid matrix and graphite particulates showed promise for peptide and protein analysis.

Purpose of the Study:

To demonstrate the utility of liquid matrix and graphite particulates for ultraviolet laser desorption/ionization (UV-LD-MS).
To investigate the mechanism, preparation, and application scope of this analytical approach.

Main Methods:

Utilized a combination of liquid matrix and graphite particulates (2 μm) for UV-LD-MS.
Investigated desorption/ionization mechanisms, preparative procedures, and analyte range.
Analyzed peptides, proteins, oligosaccharides, and synthetic polymers.

Main Results:

Achieved straightforward and general UV-LD-MS for a broad range of analytes.
Demonstrated femtomole detection sensitivities for peptides and oligosaccharides.
Graphite efficiently transfers UV energy for thermal desorption; liquid matrix aids protonation, cationization, and sample stability.

Conclusions:

The liquid matrix-graphite particulate method is a robust and versatile technique for UV-LD-MS.
This approach simplifies sample preparation and enhances analyte detection sensitivity.
The method is applicable to a wide array of compounds, including synthetic polymers with added cationizing salts.