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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

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Liposome behavior in capillary electrophoresis.

M A Roberts¹, L Locascio-Brown, W A Maccrehan

¹Chemical Sensing and Automation Technologies Group, Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899-0001, and Bioanalytical Laboratory, Institute for Comparative and Environmental Toxicology, Cornell University, Geneva, New York 14456-0462.

Analytical Chemistry

|May 31, 2011

Summary

This study explores liposome behavior in capillary electrophoresis for industrial and analytical characterization. It offers a new method for analyzing liposome properties and interactions, complementing traditional techniques.

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Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

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Published on: August 31, 2019

Area of Science:

Analytical Chemistry
Physical Chemistry
Biophysical Chemistry

Background:

Liposomes are crucial in various industrial and analytical applications.
Characterizing liposomes is essential for quality control and application development.
Traditional methods like laser light scattering have limitations in providing comprehensive characterization.

Purpose of the Study:

To investigate the electrophoretic behavior of liposomes in capillary electrophoresis (CE).
To develop CE as a method for characterizing liposomes, including their mobility and surface interactions.
To explore the potential of liposomes in analytical separations and on-capillary reactions.

Main Methods:

Capillary electrophoresis (CE) was employed to study liposome behavior under varying conditions.
Electrophoretic mobility and liposome-capillary surface interactions were characterized.
Results were compared with traditional laser light-scattering methods for size determination.
On-line and off-line experiments involving liposomes and surfactants were conducted.
Automated electrokinetic injections were used for on-capillary reactions.

Main Results:

Electrophoretic behavior of liposomes was successfully characterized using CE.
CE provided insights into liposome-capillary surface interactions.
The CE method showed comparable results to laser light-scattering for size information.
On-capillary reactions, including liposome lysis, were demonstrated.
Preliminary evidence suggests liposomes can act as a hydrophobic partitioning medium.

Conclusions:

Capillary electrophoresis offers a viable method for characterizing liposomes for industrial and analytical uses.
CE provides valuable information on electrophoretic mobility and surface interactions.
The study demonstrates the versatility of CE for on-capillary reactions and potential applications in separations.