Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of the free metal ion species in soluble nickel removal by activated sludge.

Environmental science & technology·2012

Same author

Effect of Stationary-Phase Sorption of Organic Modifier from a Water-Rich Mobile Phase on Solute Retention by an ODS Bonded Phase.

Analytical chemistry·2011

Same author

Chain Unfolding in an ODS-Bonded Phase Caused by the Sorbed Tetra-n-butylammonium Ion.

Analytical chemistry·2011

Same author

Electrical Double-Layer Effects and the Origin of Indirect UV Detection in the Liquid Chromatography of a Cationic Sample Using a Cationic Probe.

Analytical chemistry·2011

Same author

Headspace solvent microextraction.

Analytical chemistry·2002

Same author

Observation of gel-induced protein modifications in sodium dodecylsulfate [corrected] polyacrylamide gel electrophoresis and its implications for accurate molecular weight determination of gel-separated proteins by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

Journal of the American Society for Mass Spectrometry·1999

Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026

Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026

Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026

Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026

Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026

Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Solvent microextraction into a single drop.

M A Jeannot¹, F F Cantwell

¹Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.

Analytical Chemistry

|May 31, 2011

Summary

This summary is machine-generated.

This study introduces a simple, low-solvent analytical method combining solvent extraction and gas chromatography (GC). The technique offers accurate quantification with minimal solvent use, making it an efficient tool for chemical analysis.

More Related Videos

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Published on: August 26, 2016

Extraction of Plant-based Capsules for Microencapsulation Applications

Extraction of Plant-based Capsules for Microencapsulation Applications

Published on: November 9, 2016

Related Experiment Videos

Last Updated: Jun 1, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Published on: August 26, 2016

Extraction of Plant-based Capsules for Microencapsulation Applications

Extraction of Plant-based Capsules for Microencapsulation Applications

Published on: November 9, 2016

Area of Science:

Analytical Chemistry
Separation Science

Background:

Traditional solvent extraction methods often require large solvent volumes and complex apparatus.
Developing efficient and cost-effective analytical techniques is crucial for various scientific disciplines.

Purpose of the Study:

To present a novel analytical technique integrating solvent extraction with gas chromatography (GC).
To demonstrate a method with minimal solvent consumption and high analytical accuracy.

Main Methods:

A micro-extraction probe utilizing a small organic solvent drop on a Teflon rod immersed in an aqueous sample.
Gas chromatographic (GC) analysis of the extracted organic phase for quantification.
Kinetic modeling using a convective-diffusive model to describe the extraction rate.

Main Results:

The developed technique combines solvent extraction and GC analysis in a simple, inexpensive apparatus.
Extraction rates align well with a convective-diffusive kinetic model.
Achieved a relative standard deviation of 1.7% for 4-methylacetophenone extraction in 5 minutes.

Conclusions:

The described method offers a simple, cost-effective, and low-solvent approach for analytical quantification.
The technique is suitable for analyzing analytes in aqueous solutions using gas chromatography.
This method provides a practical alternative for routine analytical procedures requiring high precision.