Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 1, 2026

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Computational analysis of high-density peptide microarray data with application from systemic sclerosis to multiple

Michael Hecker1, Peter Lorenz, Felix Steinbeck

  • 1Department of Immunology, University of Rostock, Rostock, Germany. michael.hecker@rocketmail.com

Autoimmunity Reviews
|May 31, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-world treatment patterns and determinants of therapy in systemic sclerosis: findings from the German Network for SSc cohort.

Arthritis research & therapy·2026
Same author

The Nobel Prize honours the discovery of the natural barrier against autoimmunity - a breakthrough in tolerance reconstitution: how far are we from clinical impact?

EULAR rheumatology open·2026
Same author

Tocilizumab but not Siltuximab prevents systemic inflammation in a humanized mouse model.

Frontiers in immunology·2026
Same author

Machine learning-guided multimodal profiling defines perturbed immune states at the time of cancer diagnosis.

Briefings in bioinformatics·2026
Same author

Real-World Safety and Effectiveness of JAK Inhibitors in Systemic Sclerosis: A Propensity-Matched Study From the EUSTAR Cohort.

Arthritis care & research·2026
Same author

Anti-Ro/SSA and rheumatoid factor antibodies are associated with more severe lung involvement in patients with systemic sclerosis: a study from the EUSTAR database.

European journal of internal medicine·2026

Peptide microarrays identify auto-antibodies against specific protein fragments (epitopes) in autoimmune diseases. This study found unique antibodies in systemic sclerosis patients, aiding biomarker discovery for conditions like multiple sclerosis.

Area of Science:

  • Immunology
  • Biotechnology
  • Bioinformatics

Background:

  • Auto-antibodies play a key role in autoimmune disease development.
  • High-density peptide microarrays offer a method to detect antibody reactivities against numerous peptides.
  • Identifying specific epitope-antibody-recognitions (EAR) is crucial for understanding autoimmune pathophysiology.

Purpose of the Study:

  • To demonstrate the utility of peptide microarrays for identifying auto-antigens and epitopes.
  • To investigate epitope-antibody-recognitions (EAR) in systemic sclerosis (SSc) and explore applications for multiple sclerosis (MS).
  • To present bioinformatic methods for analyzing peptide microarray data.

Main Methods:

  • Utilized high-density peptide microarrays with 3235 overlapping 15mer peptides representing 24 proteins.

More Related Videos

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

Related Experiment Videos

Last Updated: Jun 1, 2026

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

  • Analyzed serum from 10 SSc patients and 10 healthy controls.
  • Applied quality control, data pre-processing, and bioinformatic filtering techniques.

    • Main Results:

    • Peptide microarray data exhibited sparse, skewed distributions with significant inter-individual variability.
    • Identified antibodies targeting three specific peptides (MGPRRRSRKPEAPRR, TPTPGPSRRGPSLGA, GPSRRGPSLGASSHQ) in SSc patients, absent in controls.
    • These peptides correspond to known linear epitopes on centromere protein A (CENPA).

    Conclusions:

    • Peptide microarrays are effective tools for identifying disease-specific auto-antibody targets and epitopes.
    • The identified peptides and the analytical approach can serve as diagnostic or prognostic biomarkers for SSc and potentially MS.
    • This technology facilitates the investigation of epitope-antibody-recognitions (EAR) in autoimmune diseases.