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Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
Published on: June 21, 2016
Developmental patterns of PPAR and RXR gene expression during spermatogenesis
Kelwyn Thomas1, Dae-Yong Sung, Xing Chen
1Department of Anatomy and Neurobiology, Morehouse School of Medicine, Atlanta, GA 30310-1495, USA. kethomas@msm.edu
Frontiers in Bioscience (Elite Edition)
|May 31, 2011
Summary
This study reveals that peroxisome proliferator-activated receptors (PPARs) and retinoid X receptors (RXRs) are developmentally expressed in testicular cells. PPAR-Gamma specifically regulates lipid metabolism genes in Sertoli cells.
Area of Science:
- Reproductive biology
- Molecular endocrinology
- Nuclear receptor signaling
Background:
- Nuclear receptors, including PPARs and RXRs, are crucial for gene regulation during development.
- Understanding their specific roles in testicular cell differentiation is essential for reproductive health.
Purpose of the Study:
- To investigate the developmental expression patterns of PPARs and RXRs in testicular somatic and germ cells.
- To elucidate the physiological function of PPAR-Gamma in rat Sertoli cells.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) on purified mouse germ and rat Sertoli cells.
- Adenoviral vector-mediated gene transfer (cDNA and RNAi) in primary rat Sertoli cells.
- Affymetrix microarray analysis for gene expression profiling.
Main Results:
- PPAR-Alpha, -Beta, and -Gamma, along with RXR-Alpha, -Beta, and -Gamma transcripts, show developmental expression in germ and Sertoli cells.
- PPAR-Gamma overexpression or knockdown in Sertoli cells altered the expression of key lipid metabolism genes.
Conclusions:
- PPARs and RXRs are dynamically expressed during testicular development, suggesting roles in cell differentiation.
- PPAR-Gamma plays a significant role in regulating lipid metabolism within Sertoli cells.
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