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Extracellular Vesicle Tissue Factor Activity Assay
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Published on: December 29, 2023

Alternatively spliced tissue factor: discovery, insights, clinical implications.

Ramprasad Srinivasan1, Vladimir Y Bogdanov

  • 1Division of Hematology/Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.

Frontiers in Bioscience (Landmark Edition)
|May 31, 2011
PubMed
Summary

The Tissue Factor (TF) gene produces two proteins: full-length TF (flTF) and alternatively spliced TF (asTF). This review explores the distinct roles and properties of the secreted asTF, a newly discovered form.

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Published on: February 14, 2017

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Hematology

Background:

  • Human Tissue Factor (TF) is crucial for blood coagulation and embryonic development.
  • The TF gene was previously thought to produce only one protein, the integral membrane glycoprotein flTF.
  • Recent discoveries reveal the TF gene also produces alternatively spliced TF (asTF).

Purpose of the Study:

  • To review the current understanding of alternatively spliced TF (asTF) biology.
  • To discuss the structural and functional characteristics of asTF.
  • To explore the emerging field of regulated TF pre-mRNA processing.

Main Methods:

  • Literature review of studies on asTF.
  • Analysis of structural differences between flTF and asTF.
  • Discussion of experimental evidence for asTF's biologic roles.

Main Results:

  • Tissue Factor (TF) gene yields two distinct proteins: full-length TF (flTF) and alternatively spliced TF (asTF).
  • Alternatively spliced TF (asTF) lacks a transmembrane domain, enabling its secretion.
  • asTF possesses unique structural and functional characteristics separate from flTF.

Conclusions:

  • The discovery of asTF significantly expands our understanding of TF biology.
  • asTF's secreted nature suggests novel roles in biological processes.
  • Further research into asTF and its regulation is warranted.