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Isolation and characterization of 20 microsatellite loci for laurel species (Laurus, Lauraceae)
Juan M Arroyo1, Cristina Rigueiro, Rocío Rodríguez
1Department of Integrative Ecology, Estación Biológica de Doñana (CSIC), Avda. Américo Vespucio, s/n, E-41092 Sevilla, Spain.
Premise Of The Study:
Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds.
Methods And Results:
Twenty polymorphic nuclear microsatellite markers were developed using CA, GA, AAC, and ATG n-enriched genomic libraries. Given the tetraploidy of the sampled populations, we analyzed our data both as dominant loci and as codominant genotypic data to calculate allele frequencies and genetic diversity. A total of 196 and 222 alleles were found in 37 Mediterranean (L. nobilis) and 26 Macaronesian islands (L. azorica) individuals, respectively.
Conclusions:
Levels of polymorphism of the reported markers are adequate for studies of diversity and parentage in natural populations of this Tertiary relict tree.
